Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: RNA-based micelles: A novel platform for paclitaxel loading and delivery

doi: 10.1016/j.jconrel.2018.02.014

Figure Lengend Snippet: A. Assay cytotoxicity effects of pRNA-3WJ-PTX micelles by MTT assay. B. Assay apoptotic effects of pRNA-3WJ-PTX micelles by PI/Annexin V-FITC dual staining and FACS analysis. C. Caspase-3 assay.

Article Snippet: Apoptosis study in in vitro cell model FITC Annexin V Apoptosis Detection Kit (BD Pharmingen) and Caspase-3 Assay Kit (BD Pharmingen) were used as previously reported [ 64 ] to study cell apoptosis induced by pRNA-3WJ-PTX micelles treatment.

Techniques: MTT Assay, Staining, Caspase-3 Assay

Journal: iScience

Article Title: Human OPN-derived peptide SV prevents BPD model through interacting with KIF5B to repair mitochondrial damage and inhibit apoptosis

doi: 10.1016/j.isci.2025.113691

Figure Lengend Snippet: SV repairs LPS-induced cell damage in vitro may rely on the inhibition of apoptosis (A) The CCK8 assay detected the proliferation of cells in the control, LPS (100 ng/mL), and LPS+SV (100 μM) groups, N = 3. (B) Flow assay of ROS levels in MLE-12 cells from control, LPS, and LPS+SV groups, N = 3. (C) Apoptosis of MLE-12 cells in the control, LPS, and LPS+SV groups was detected using flow cytometry, N = 3. (D) qPCR analysis showed the mRNA levels of Bcl-2, Bax, SPC, IL-1β, and IL-6, N = 6. (E and F) Western blot revealed the protein expression of Bcl-2, Bax, SPC, and VEGFA (vascular endothelial growth factor) in MLE-12 cells of in three groups, N = 3. Unpaired t test, ∗/#/ p < 0.05,∗∗/##/ p < 0.01, and ∗∗∗/ p < 0.001. CTL, control; LPS, BPD induced by lipopolysaccharide.

Article Snippet: Apoptosis was evaluated using an Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, China).

Techniques: In Vitro, Inhibition, CCK-8 Assay, Control, Flow Cytometry, Western Blot, Expressing

Journal: iScience

Article Title: Human OPN-derived peptide SV prevents BPD model through interacting with KIF5B to repair mitochondrial damage and inhibit apoptosis

doi: 10.1016/j.isci.2025.113691

Figure Lengend Snippet: SV repairs hyperoxia-induced cell damage in vitro may rely on the inhibition of apoptosis (A) The CCK8 assay detected the proliferation of cells in the control, hyperoxia (Hyp), and hyperoxia with SV (100 μM) groups, N = 3. (B) Flow assay of ROS levels in MLE-12 cells from control, Hyp, and Hyp+SV groups, N = 3. (C) Apoptosis of MLE-12 cells in the control, Hyp, and Hyp+SV groups was detected using flow cytometry, N = 3. (D) mRNA expression of Bcl-2, Bax, SPC, IL-1β, and IL-6 in three groups of MLE-12 cells, N = 6. (E and F) Western blot revealed the protein expression of Bcl-2, Bax, SPC, and VEGFA in MLE-12 cells of in three groups, N = 3. Unpaired t test, ∗/#/ p < 0.05,∗∗/##/ p < 0.01, and ∗∗∗/###/ p < 0.001. CTL, control; Hyp, BPD induced by hyperoxia.

Article Snippet: Apoptosis was evaluated using an Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, China).

Techniques: In Vitro, Inhibition, CCK-8 Assay, Control, Flow Cytometry, Expressing, Western Blot

Journal: iScience

Article Title: Human OPN-derived peptide SV prevents BPD model through interacting with KIF5B to repair mitochondrial damage and inhibit apoptosis

doi: 10.1016/j.isci.2025.113691

Figure Lengend Snippet: SV improves the LPS-induced BPD animal model (A) Body weight changes from 1 to 7 days in control, LPS, and LPS+SV groups of mice, N = 6. (B) Lung morphology of control, LPS, and LPS+SV groups, N = 6. (C) Lung histopathologic changes (H&E), N = 6. Mean linear intercept (MLI) quantitative represents the mean airspace diameter and mean alveolar number (MAN) quantitative represents the mean number of alveoli. Scale bars: 500 μm/100 μm. (D) Representative images of TUNEL immunostaining showing the apoptosis (green staining) in rat lung tissue of control, LPS and LPS+SV groups, N = 6. Representative images of Ki67 immunostaining showing the proliferation (red staining) in rat lung tissue of control, LPS, and LPS+SV groups; N = 6. Scale bars: 500 μm. (E) qPCR analysis showed the mRNA levels of Bcl-2, Bax, SPC, IL-1β, and IL-6, N = 6. (F and G) Western blot showed the protein levels of Bcl-2, Bax, SPC, and VEGFA, N = 6. Unpaired t test, ∗/#/ p < 0.05,∗∗/##/ p < 0.01, and ∗∗∗/###/ p < 0.001. CTL, control; LPS, BPD induced by lipopolysaccharide. The LPS concentration was 500 μg/kg and the SV intervention concentration was 200 μg/kg depending on the weight of the pups per day.

Article Snippet: Apoptosis was evaluated using an Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, China).

Techniques: Animal Model, Control, TUNEL Assay, Immunostaining, Staining, Western Blot, Concentration Assay

Journal: iScience

Article Title: Human OPN-derived peptide SV prevents BPD model through interacting with KIF5B to repair mitochondrial damage and inhibit apoptosis

doi: 10.1016/j.isci.2025.113691

Figure Lengend Snippet: SV improves the hyperoxia-induced BPD animal model (A) Body weight changes from 1 to 7 days in control, Hyp, and Hyp+SV groups of mice, N = 6. (B) Lung morphology of control, Hyp, and Hyp+SV groups of mice, N = 6. (C) Lung histopathologic changes (H&E), N = 6. MLI quantitative represents the mean airspace diameter and MAN quantitative represents the mean number of alveoli. Scale bars: 500 μm/100 μm. (D) Representative images of TUNEL immunostaining showing the apoptosis (green staining) in rat lung tissue of control, Hyp, and Hyp+SV groups. Representative images of Ki67 immunostaining showing the proliferation (red staining) in rat lung tissue of control, LPS, and LPS+SV groups, N = 6. Scale bars: 500 μm. (E) qPCR analysis showed the mRNA levels of Bcl-2, Bax, SPC, IL-1β, and IL-6, N = 6. (F and G) Western blot showed the protein levels of Bcl-2, Bax, SPC, and VEGFA, N = 6. Unpaired t test, ∗/#/ p < 0.05,∗∗/##/ p < 0.01, and ∗∗∗/###/ p < 0.001. CTL, control; Hyp, BPD induced by hyperoxia. The SV intervention concentration was 200 μg/kg depending on the weight of the pups per day.

Article Snippet: Apoptosis was evaluated using an Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, China).

Techniques: Animal Model, Control, TUNEL Assay, Immunostaining, Staining, Western Blot, Concentration Assay

Journal: World Journal of Surgical Oncology

Article Title: Circ_0026416 downregulation blocks the development of colorectal cancer through depleting MYO6 expression by enriching miR-545-3p

doi: 10.1186/s12957-021-02407-y

Figure Lengend Snippet: Circ_0026416 downregulation inhibited CRC development in vitro and in vivo. A The expression of circ_0026416 in HCT-8 and SW480 cells after si-circ_0026416 transfection was checked by qPCR. B , C Cell proliferation was checked by CCK-8 assay. D Cell proliferation was also checked by colony formation assay. E , F Cell migration and cell invasion were examined using Transwell assay. G The ability of angiogenesis was checked by tube formation assay. H Cell apoptosis was examined using flow cytometry assay. I , J The protein levels of E-Cadherin, vimentin, and N-Cadherin were determined by western blot. K – M Animal study was performed to determine the role of circ_0026416 knockdown in vivo. N The expression of circ_0026416 in tumor tissues from animal model was checked by qPCR. * P < 0.05

Article Snippet: At 48 h post-transfection, the Annexin V-FITC Apoptosis Detection Kit (Beyotime, Shanghai, China) was used to monitor cell apoptosis using a flow cytometer (BD Biosciences) according to the protocol from kit.

Techniques: In Vitro, In Vivo, Expressing, Transfection, CCK-8 Assay, Colony Assay, Migration, Transwell Assay, Tube Formation Assay, Flow Cytometry, Western Blot, Knockdown, Animal Model

Journal: World Journal of Surgical Oncology

Article Title: Circ_0026416 downregulation blocks the development of colorectal cancer through depleting MYO6 expression by enriching miR-545-3p

doi: 10.1186/s12957-021-02407-y

Figure Lengend Snippet: Circ_0026416 knockdown inhibited CRC cell development by enriching miR-545-3p. In function rescue experiments, A , B cell proliferation was assessed by CCK-8 assay. C Colony formation assay was performed to monitor cell proliferation. D , E Cell migration and cell invasion were determined by Transwell assay. F The ability of angiogenesis was checked by tube formation assay. G Flow cytometry assay was conducted to assess cell apoptosis. H , I The protein levels of E-Cadherin, vimentin, and N-Cadherin were quantified by western blot. * P < 0.05

Article Snippet: At 48 h post-transfection, the Annexin V-FITC Apoptosis Detection Kit (Beyotime, Shanghai, China) was used to monitor cell apoptosis using a flow cytometer (BD Biosciences) according to the protocol from kit.

Techniques: Knockdown, CCK-8 Assay, Colony Assay, Migration, Transwell Assay, Tube Formation Assay, Flow Cytometry, Western Blot

Journal: World Journal of Surgical Oncology

Article Title: Circ_0026416 downregulation blocks the development of colorectal cancer through depleting MYO6 expression by enriching miR-545-3p

doi: 10.1186/s12957-021-02407-y

Figure Lengend Snippet: MiR-545-3p restoration blocked CRC cell malignant behaviors by targeting MYO6. In function rescue experiments, A , B cell proliferation was assessed by CCK-8 assay. C Cell proliferation was assessed by colony formation assay. D , E Cell migration and cell invasion were examined using Transwell assay. F The ability of angiogenesis was checked by tube formation assay. G Flow cytometry assay was conducted to assess cell apoptosis. H , I The protein levels of E-Cadherin, vimentin and N-Cadherin were quantified by western blot. * P < 0.05

Article Snippet: At 48 h post-transfection, the Annexin V-FITC Apoptosis Detection Kit (Beyotime, Shanghai, China) was used to monitor cell apoptosis using a flow cytometer (BD Biosciences) according to the protocol from kit.

Techniques: CCK-8 Assay, Colony Assay, Migration, Transwell Assay, Tube Formation Assay, Flow Cytometry, Western Blot